Fasciola hepatica
Title: One-Health Approach to Study Human Fasciola hepatica Transmission and Inform Strategic Control (Copy 1)
Year Started: 2022
Cross sectional study including human, livestock, snails, and the environment to study the transmission of Fasciola hepatica in the highlands of Peru. Feces (human and livestock), serum (human), plasma (human), and PBMCs (human), tissue (snail), and water samples will be collected to study factors associated with transmission. We expect to include 1,500 children and adults and their farms (water bodies within them). We will study 4,800 livestock.
- Access:
- Open only through collaboration
- Type:
- Cross-sectional
- Status:
- In progress
- Consent restrictions:
- No restrictions
| Associated Data Type | Procurement Timeframe |
|---|---|
| Followup records | 0 - 3 months |
| Freezer temperature logs | 3 - 6 months |
| Survey data | 0 - 3 months |
| Treatment records | 0 - 3 months |
Both sexes
Child (6 - 12 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Stool | -85°C to -60°C | Not applicable | 75% -99% |
Both sexes
Adolescent (12 - 18 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Stool | -85°C to -60°C | Not applicable | 75% -99% |
Both sexes
Young child (2 - 6 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Stool | -85°C to -60°C | Not applicable | 75% -99% |
Both sexes
Young adult (18 - 40 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Stool | -85°C to -60°C | Not applicable | 75% -99% |
Both sexes
Adult (> 40 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Stool | -85°C to -60°C | Not applicable | 75% -99% |
Both sexes
Young child (2 - 6 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Serum | -85°C to -60°C | Not applicable | 51 - 75% |
Both sexes
Child (6 - 12 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Serum | -85°C to -60°C | Not applicable | 51 - 75% |
Both sexes
Adolescent (12 - 18 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Serum | -85°C to -60°C | Not applicable | 51 - 75% |
Both sexes
Young adult (18 - 40 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Serum | -85°C to -60°C | Not applicable | 51 - 75% |
Both sexes
Young child (2 - 6 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Plasma (single spun) | -85°C to -60°C | Not applicable | 75% -99% |
Both sexes
Adolescent (12 - 18 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Plasma (single spun) | -85°C to -60°C | Not applicable | 75% -99% |
Both sexes
Young adult (18 - 40 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Plasma (single spun) | -85°C to -60°C | Not applicable | 75% -99% |
Both sexes
Adult (> 40 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Plasma (single spun) | -85°C to -60°C | Not applicable | 75% -99% |
Both sexes
Adolescent (12 - 18 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Isolated peripheral blood mononuclear cells (viable) | Liquid nitrogen | Not applicable | 26 - 50% |
Fasciola hepatica
Title: One-Health Approach to Study Human Fasciola hepatica Transmission and Inform Strategic Control (Copy 1) (Copy 1)
Year Started: 2022
Cohort study including human, livestock, snails, and the environment to study the transmission of Fasciola hepatica in the highlands of Peru. Feces (human and livestock), serum (human), plasma (human), and PBMCs (human), tissue (snail), and water samples will be collected to study factors associated with transmission. We expect to include 500 children and adults and their farms (water bodies within them). We will study 4,800 livestock.
- Access:
- Open only through collaboration
- Type:
- Cohort
- Status:
- Not-started
- Consent restrictions:
- Human genetic analysis restriction
| Associated Data Type | Procurement Timeframe |
|---|---|
| Followup records | 0 - 3 months |
| Freezer temperature logs | 3 - 6 months |
| Survey data | 0 - 3 months |
| Treatment records | 0 - 3 months |
Both sexes
Child (6 - 12 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Stool | -85°C to -60°C | Not applicable | 75% -99% |
Both sexes
Adolescent (12 - 18 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Stool | -85°C to -60°C | Not applicable | 75% -99% |
Both sexes
Child (6 - 12 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Serum | -85°C to -60°C | Not applicable | 51 - 75% |
Both sexes
Adolescent (12 - 18 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Serum | -85°C to -60°C | Not applicable | 51 - 75% |
Both sexes
Adolescent (12 - 18 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Plasma (single spun) | -85°C to -60°C | Not applicable | 75% -99% |
Both sexes
Adolescent (12 - 18 years)
101 - 500 donors
| Material Type | Extraction Procedure | Storage Temperature | Preservation Type | Microscopic Assessment | % of Sample Set |
|---|---|---|---|---|---|
| Isolated peripheral blood mononuclear cells (viable) | Liquid nitrogen | Not applicable | 26 - 50% |